Slime Secretion from Physarum Plasmodium

All three images are of the same Physarum plasmodium. The left hand panel is a DIC (Nomarski) image; the right hand panel shows an immunofluorescence image after labelling with a polyclonal antibody for slime; the central panel shows the two (left/right) panels after pseudocoloring (green = DIC, red = slime) and merging. The inset shows the close correlation of slime and small surface projections. Scale bars show 10 um units.
Image courtesy of Dr. Satoshi Ogihara. For more information, contact Satoshi Ogihara

References:

1. Sesaki,H. and Ogihara,S. (1997): Secretion of Slime, the Extracellular Matrix of the Plasmodium, as Visualized with a Fluorescent Probe and Its Correlation with Locomotion on the Substratum. Cell Structure and Function, 22:279-289.
2. Sesaki,H. and Ogihara,S. (1997):Protrusion of cell surface coupled with single exocytotic events of secretion of the slime in Physarum plasmodia. J. Cell Science, 110:809-818.