Introduction
Section A: Choice of Strains
Heterothallic and apogamic strains
Genetic heterogeneity among strains
Finding the strain you need
Section B: Culturing the Vegetative Stages
Culture methods for plasmodia
Culture methods for amoebae
Storage of stock cultures
Incubation temperatures
Changes in genetic constitution of amoebae during culture
Changes in plasmodia during culture
Section C: Developmental Changes and Cellular Transformations
Encystment
The amoeba-flagellate transformation
The amoebal-plasmodial transition
Section D: Genetic Analysis
Mutants
Genetic analysis of a newly-isolated mutant
Strain construction and linkage analysis
Dominance and complementation tests
Section E: DNA Transformation
References