Stage II RAFT
This video sequence was recorded (and displayed) in real time, using Nomarski (DIC) optics. The full frame width is 56 um. The cell gradually extends a flagellum (it is just barely detectable on the left side of the cell as this sequence begins), which becomes increasingly active. Among the movements displayed by such cells is the slow "migration" of the basal body (from which the flagellum is growing) around the perimeter of the cell.
STAGE II (videosequence B): Cells at stage II of the transformation have grown a flagellum but otherwise are morphologically similar to those of stage I. The flagellum that is first recognized corresponds to the long, anteriorly-directed one of the two that are eventually formed. It seems to shift its position in that it appears at different points around the cell periphery; the nucleus, which is associated with the basal bodies via microtubular arrays, often follows the flagellum as it changes position. Stage II cells comprise a major group in a transforming population for only a very brief time.
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Last modified:Friday, December 5, 2003
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