Institute of Medical Chemistry and Biochemistry, University of Innsbruck, Fritz-Pregl-Strasse 3, A-6020 Innsbruck, Austria
Screening various Physarum mutants with altered sporulation behaviour generated from the wild-type strain CS310 by UV-irradiation (kindly provided by W. Marwan, University of Freiburg, Germany), we found that all of them produced NO and cGMP with the exception of CS114, a mutant unable to sporulate. This mutant formed NO but lacked the rise in cGMP observed in the other strains. Accordingly, CS114 also had an impaired induction of guanylate cyclase activity over the starvation period preceding sporulation that was comparable to the effect of inhibiting NOS with NIL in the wild-type CS310. These data substantiate our work that defined NO/cGMP signalling as a requirement for sporulation of Physarum (1).
(1) Golderer G et al. (2001) Genes & Dev. 15, 1299-1309.
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Last modified: Tuesday, December 3, 2002