Double-Labelled Physarum MicroplasmodiumFragminP and F-actin

Image of microplasmodium of Physarum polycephalum acquired by confocal microscopy. Double labelling for fragminP (red, indirect immunofluorescence) and actin (green, FITC-phalloidin). Note that the plasmodium-specific fragmin does not colocalize with the fibrillar actin network; instead the fragminP is found in small surface projections that contain relatively little F-actin.
Image courtesy of Bruno Constantin. For more information, contact Jan Gettemans

Reference:

T'Jampens,D. et. al. (1997): Molecular Cloning, Over-Expression, Developmental Regulation and Immunolocalization of FragminP, a Gelsolin-related Actin-binding Protein from Physarum polycephalum Plasmodia. J. Cell Science, 110:1215-1226.